Radioimmunoassay of 2-hydroxyestrone in urine
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay of 2-hydroxyestrone in urine.
Catechol estrogens such as 2-OH estrone are interesting estrogen metabolites formed in several human tissues and excreted in urine. We developed and thoroughly validated a radioimmunoassay for urinary 2-OH estrone that has several advantages over published RIAs. Because we developed a relatively specific antiserum, we did not include a preliminary chromatographic step to eliminate cross-reactin...
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A sensitive RIA for benzoylecgonine in body fluids is described. The label is radioiodinated p-hydroxybenzoylecgonine. The antiserum from an Emit-dau cocaine metabolite kit (Syva) is used at a 1:600 dilution. The standards are benzoylecgonine in buffer. PEG-6000 is used to separate the bound and free fractions. Cocaine, ecgonine, and methylecgonine have cross-reactivities of 2%, 1%, and 0.1%, r...
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Rapid and simple enzyme immunoassays (EIAs) were recently developed to measure 2-hydroxyestrone and 16alpha-hydroxyestrone in unextracted urine. The balance between these competing estrogen metabolism pathways may serve as a biomarker of breast cancer risk. Before testing these assays in epidemiologic studies, we evaluated their reproducibility, and validity relative to gas chromatography-mass ...
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BACKGROUND It has been suggested that women who metabolize a larger proportion of their endogenous estrogen via the 16alpha-hydroxylation pathway may be at elevated risk of breast cancer compared with women who metabolize proportionally more estrogen via the 2-hydroxylation pathway. However, the supporting epidemiologic data are scant. Consequently, we compared the ratio of urinary 2-hydroxyest...
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Experimental studies have shown that two main estrogen metabolites hydroxylated by CYP1A1 and CYP1B1 in the breast differentially affect breast cell proliferation and carcinogenesis. Although 16α-hydroxyestrone (16αOHE1) exerts estrogenic activity through covalent estrogen receptor (ER) binding, 2-hydroxyestrone (2OHE1) presumably has antiestrogenic capabilities. The ratio of 2OHE1 to 16αOHE1 r...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1994
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/40.1.80